Basophils and mast cells (MCs) are important effector cells in the immune system. For a long time, it has been known that these cells can be activated though the cross-linking of IgE antibodies bound to their high-affinity receptor (FcεRI). However, evidence has accumulated suggesting that these cells can also be activated by various IgE-independent mechanisms. Occupation of MAS Related GPR Family Member X2 (MRGPRX2), a G protein-coupled receptor, is described as an alternative IgE-independent activation mechanism. Here we describe a flow cytometric technique to analyze MRGPRX2 expression and its functionality on cultured human MCs and conditioned basophils, that is, basophils with upregulated surface expression of MRGPRX2.
CITATION STYLE
Elst, J., Sabato, V., Hagendorens, M. M., van Houdt, M., Faber, M. A., Bridts, C. H., … Mertens, C. (2020). Measurement and functional analysis of the mas-related G protein-coupled receptor MRGPRX2 on human mast cells and basophils. In Methods in Molecular Biology (Vol. 2163, pp. 219–226). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0696-4_18
Mendeley helps you to discover research relevant for your work.