The acute murine lung infection model monitors Pseudomonas aeruginosa airway infections by multiple continuous and endpoint parameters. After intratracheal or intranasal infection it characterizes the course of infection via head-out spirometry, rectal temperature, weight loss, a body condition score based on nine physiological parameters, lung bacterial numbers, organ dissemination of bacteria, and a semiquantitative assessment of lung inflammation and further analysis. The generated data allows a robust classification of virulence of mutant or wild-type P. aeruginosa strains and/or of the susceptibility of wild-type or engineered mouse strains to infection. If standardized, the model is applicable to the preclinical assessment of antipseudomonal prevention and intervention strategies.
CITATION STYLE
Munder, A., & Tümmler, B. (2014). Assessing Pseudomonas virulence using mammalian models: Acute infection model. Methods in Molecular Biology, 1149, 773–791. https://doi.org/10.1007/978-1-4939-0473-0_59
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