Genotypic variation in virulence level of several brown planthopper (Nilaparvata lugens stål) populations of rice (oryza sativa l.)

Abstract

Background and Objective: The virulence level information of the Brown Planthopper (BPH) population is required to determine which populations will be used in the rice breeding program. This is to give a high pressure on the screening lines to obtain highly resistant lines. The purpose of this study was to determine the virulence level of 5 BPH populations and to determine resistant varieties to BPH populations that can be used as donor lines. Materials and Methods: The study used the Standard Seedbox Screening Test method on 5 BPH populations (Kediri, Subang, Banyuasin, Klaten and South Lampung). The genotype used in this study consisting of 9 BPH differential and 5 commercial rice varieties. The BPH population variance was analyzed and then grouped based on the level of virulence in each genotype using the UPGMA method. Results: Klaten's BPH population had the highest virulence (5.95), meanwhile, the BPH population from Subang (4.86) had the lowest virulence. Based on the clustering analysis, BPH populations from Kediri, Banyuasin and South Lampung are in the same group. Meanwhile, the BPH population from Klaten and Subang has been clustered in the different groups each. Balamawee identified as a high and stable resistance variety to all tested BPH populations followed by Rathu Heenati, PTB33, Swarnalata, Inpari13 and IR64 with medium resistance. Conclusion: The BPH population from Klaten is recommended to be used in the lines screening program because of its high virulence level and Balamawee is identified as a potential donor to develop BPH resistant variety.