Sero-prevalence and risk factors of Brucella presence in farm bulk milk from open and zero grazing cattle production systems in Rwanda

Abstract

Background: Animal and human brucelloses have been reported in Rwanda, human brucellosis being linked to drinking inadequately heat-treated milk. However, information on Brucella detection and prevalence in milk produced in Rwanda is limited. Objectives: To determine the sero-prevalence and risk factors of Brucella in farm bulk milk from zero and open grazing cattle production systems in Rwanda. Methods: A total of 330 farm bulk milk samples were collected from 198 zero grazing farms and 132 open grazing farms in a cross-sectional study in Rwanda. Sero-prevalence of Brucella in milk was analysed using indirect enzyme-linked immunosorbent assay. A questionnaire was administered to farmers to determine the risk factors of milk contamination with Brucella. Results: Anti-Brucella antibodies were prevalent in 19.7% (95% confidence interval (CI), 15.5–24.4) of the 330 collected farm bulk milk. Sero-prevalence was significantly higher (p < 0.05) in open grazing farms (37.9% [50/132]) than in zero grazing farms (7.6% [15/198]). Practising open grazing system (odds ratio, OR = 69.5; 95% CI = 1.6–3033.6), history of abortion (OR = 19.5; 95% CI = 8.1–46.8) and placenta retention (OR = 4.2; 95% CI = 1.7–10.3) were the significant risk factors for the presence of anti-Brucella antibodies in milk. Conclusion: Notably, more than a third of farm bulk milk from open grazing farms in Rwanda contains Brucella antibodies. Considering the zoonotic nature of Brucella, there is a need to reinforce brucellosis control programs in the country.