Sirtuins are a family of proteins with NAD + -dependent deacetylase or mono-ADP-ribosyltransferase activity. SIRT1, the mammalian ortholog most closely related to Sir2 (the first gene of this family discovered in yeast), exhibits anti-senescence activity in a wide range of mammalian cells. Here, we describe the use of an ex vivo senescence model to study SIRT1 function in primary endothelial cells isolated from the porcine aorta. The methods can be applied to the investigation of the role of SIRT1 in the development of endothelial senescence and atherosclerosis. © Springer Science+Business Media New York 2013.
CITATION STYLE
Bai, B., & Wang, Y. (2013). Methods to investigate the role of SIRT1 in endothelial senescence. Methods in Molecular Biology, 965, 327–339. https://doi.org/10.1007/978-1-62703-239-1_22
Mendeley helps you to discover research relevant for your work.