Real-time in vivo imaging of adult Zebrafish brain using optical coherence tomography

Abstract

We report noninvasive imaging of the brain of adult Zebrafish (Danio rerio) using real time optical coherence tomography (OCT) capable of acquiring cross sectional 2D OCT images @ 8 frames/sec. Anatomic features such as telencephalon, tectum opticum, eminentia Granularis and cerebellum were clearly resolved in the OCT images. A 3D model of Zebrafish brain was reconstructed, for the first time to our knowledge, using these 2D OCT images. © 2009 by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.