Bacillus subtilis is a critical host for producing recombinant proteins. However, the SDS-PAGE process, including the sample preparation steps, varies among B. subtilis-related studies, making it impossible to compare findings. Hence, this paper provides a simple guide to culture and collect B. subtilis cells through an OD600 measurement and a protocol for SDS-PAGE. These techniques were applied to check the expression of a BgaB, a reporter protein and LukF-PV, a potential vaccine candidate against S. aureus, in the cytoplasm of B. subtilis under the control of a strong promoter, Pgrac212. This protocol could be helpful for scientists in preparing samples and generating an SDS-PAGE experiment, as well as favoring the unification of research about protein expression in B. subtilis.
CITATION STYLE
Dao, T. T. M., Truong, D. D., Duong, L. N. H., Nguyen, N. N. Y., & Nguyen, H. D. (2023). Preparation of Bacillus subtilis cell samples and generation of an SDS-PAGE. BioTechniques, 74(3), 123–129. https://doi.org/10.2144/btn-2022-0083
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