Selection of lead antibodies from naive ribosome display antibody libraries

Abstract

A large antibody fragment library (>10 12) has been generated in ribosome display format. The library was constructed in a two-step process. First, variable (V) genes were isolated from human B cells from a panel of 14 donors and cloned into designated ribosome display vectors to create a gene bank. Second, RD-VH and RD-VL genes from individual immunoglobulin families were combined in vitro resulting in 112 scFv ribosome display sub-libraries. These were subsequently pooled to form a master library. This library was used to isolate a panel of antibodies to the IL4 receptor by three rounds of selections on a soluble target. © 2012 Springer Science+Business Media, LLC.