Reliable detection and quantification of antigen-specific T cells are critical for assessing the immunogenicity of vaccine candidates. In this chapter, we describe the use of ELISpot and flow cytometry-based assays for efficient detection, mapping, and functional characterization of memory T lymphocytes in different tissues of rhesus macaques immunized with plasmid DNA. Flow cytometric assays provide a large amount of information, both phenotypic and functional, about individual cells, while the ELISpot is well suited for high throughput sample screening.
CITATION STYLE
Hu, X., Felber, B. K., & Valentin, A. (2021). Assessing Antigen-Specific Cellular Immune Responses upon HIV /SIV Plasmid DNA Vaccination in the Nonhuman Primate Model. In Methods in Molecular Biology (Vol. 2197, pp. 113–131). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0872-2_6
Mendeley helps you to discover research relevant for your work.