The IncA/C plasmids are broad host-range vehicles which have been associated with wide dissemination of CMY-2 among Enterobacteriaceae of human and animal origins. Acquired metallo-β-lactamases (MBLs) such as the IMP-type enzymes are increasingly reported in multidrug-resistant Gram-negative bacteria worldwide, particularly in Enterobacteriaceae. We described the complete sequence of the first IMP-4-encoding IncA/C2 plasmid, pIMP-PH114 (151,885 bp), from a sequence type 1 Klebsiella pneumoniae strain that was recovered from a patient who was hospitalized in the Philippines. pIMP-PH114 consists of a backbone from the IncA/C2 plasmids, with the insertion of a novel Tn21-like class 1 integron composite structure (containing the cassette array blaIMP-4-qacG-aacA4-catB3, followed by a class C β-lactamase blaDHA-1 and the mercury resistance operon, mer-RTPCADE) and a sul2-floR encoding region. Phylogenetic analysis of the IncA/C repA sequences showed that pIMP-PH114 formed a subgroup with other IncA/C plasmids involved in the international spread of CMY-2, TEM-24 and NDM-1. Identical blaIMP-4 arrays have been described among different Enterobacteriaceae and Acinetobacter spp. in China, Singapore and Australia but the genetic context is different. The broad host range of IncA/C plasmids may have facilitated dissemination of the blaIMP-4 arrays among different diverse groups of bacteria. © Springer Science+Business Media 2013.
CITATION STYLE
Ho, P. L., Lo, W. U., Chan, J., Cheung, Y. Y., Chow, K. H., Yam, W. C., … Que, T. L. (2014). pIMP-PH114 carrying blaIMP-4in a Klebsiella pneumoniae strain is closely related to other multidrug-resistant IncA/C2 plasmids. Current Microbiology, 68(2), 227–232. https://doi.org/10.1007/s00284-013-0471-x
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