Mass cytometry time-of-flight (CyTOF) empowers us to understand acute myeloid leukemia (AML) biology at the single-cell level. This technology, combined with advanced data-analysis methods, enables identification and characterization of the rare AML stem/progenitor cells that play key roles in drug resistance and AML relapse. Here we provide a protocol for AML sample preparation for CyTOF and keynotes emphasizing critical steps in and troubleshooting strategies for this procedure.
CITATION STYLE
Zeng, Z., Konopleva, M., & Andreeff, M. (2017). Single-cell mass cytometry of acute myeloid leukemia and leukemia stem/progenitor cells. In Methods in Molecular Biology (Vol. 1633, pp. 75–86). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7142-8_5
Mendeley helps you to discover research relevant for your work.