Methyl jasmonate elicitation enhances glycyrrhizin production in glycyrrhiza inflata hairy roots cultures

Abstract

Hairy roots were induced by infecting stems and leaves of Glycyrrhiza infl ata with Agro-bacterium rhizogenes ATCC 15834. The optimization of growth and glycyrrhizin accumulation of G. inflata hairy roots was studied. Sucrose (6%, w/v) was optimal for growth and glycyrrhizin accumulation in G. inflata hairy roots. Effects of elicitors like chitosan, methyl jasmonate, and yeast extract on glycyrrhizin production were studied. Methyl jasmonate (100 μm) was most efficient in enhancing glycyrrhizin production up to almost 109 μg/g dry weight on day 5 of elicitation. These results indicate that application of elicitors can enhance the capacity of G. infl ta hairy roots to produce glycyrrhizin. © 2011 Verlag der Zeitschrift für Naturforschung.