Genotyping of strawberry (Fragaria × ananassa Duch.) cultivars by DNA markers: Interlaboratory study

Abstract

Fourteen Japanese laboratories validated the reproducibility of genotyping by 25 cleavage amplified polymorphic sequence (CAPS) markers for discrimination of strawberry (Fragaria × ananassa Duch.) cultivars. Both the sensitivity and specificity rate of 12 markers were 100%, those of another 12 were >95%, and those of 1 were >90%. These results indicate that the method of genotyping by the CAPS markers was highly reproducible and could provide a useful basis for practical identification of strawberry cultivars. This is the first report of the statistical validation of crop genotyping by DNA markers.