Indirect coculture of stem cells with fetal chondrons using PCL electrospun nanofiber scaffolds

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Abstract

In vitro coculture system provides a powerful tool for tissue engineering. In this study, we evaluated the gene expressions of human adipose-derived stem cells (ASCs) on polycaprolactone (PCL) scaffold in coculture model with fetal chondrons. Electrospun PCL scaffolds (900 nm fiber diameter) were created and human infrapatellar fat pad-adipose-derived stem cells (IPFP-ASCs) were seeded on these scaffolds. Scanning electron microscopy (SEM) showed attachment of human IPFP–ASCs to scaffold. IPFP-ASCs on scaffolds were cocultured with fetal chondrons in transwell. Gene expressions were investigated using real-time polymerase chain reaction (real-time PCR). In comparison with control group, the expression level of collagen type 2 and aggrecan were significantly decreased but Indian Hedgehog(IHH) significantly increased (P < 0.05).These findings may interpreted that IPFP-ASCs seeded on PCL scaffold, in cocultures with fetal chondrons are tending toward osteogenesis rather than chondrogenesis.

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Nikpou, P., Soleimani Rad, J., Mohammad Nejad, D., Samadi, N., Roshangar, L., Navali, A. M., … Soleimani Rad, S. (2017). Indirect coculture of stem cells with fetal chondrons using PCL electrospun nanofiber scaffolds. Artificial Cells, Nanomedicine and Biotechnology, 45(2), 283–290. https://doi.org/10.3109/21691401.2016.1146733

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