In vitro coculture system provides a powerful tool for tissue engineering. In this study, we evaluated the gene expressions of human adipose-derived stem cells (ASCs) on polycaprolactone (PCL) scaffold in coculture model with fetal chondrons. Electrospun PCL scaffolds (900 nm fiber diameter) were created and human infrapatellar fat pad-adipose-derived stem cells (IPFP-ASCs) were seeded on these scaffolds. Scanning electron microscopy (SEM) showed attachment of human IPFP–ASCs to scaffold. IPFP-ASCs on scaffolds were cocultured with fetal chondrons in transwell. Gene expressions were investigated using real-time polymerase chain reaction (real-time PCR). In comparison with control group, the expression level of collagen type 2 and aggrecan were significantly decreased but Indian Hedgehog(IHH) significantly increased (P < 0.05).These findings may interpreted that IPFP-ASCs seeded on PCL scaffold, in cocultures with fetal chondrons are tending toward osteogenesis rather than chondrogenesis.
CITATION STYLE
Nikpou, P., Soleimani Rad, J., Mohammad Nejad, D., Samadi, N., Roshangar, L., Navali, A. M., … Soleimani Rad, S. (2017). Indirect coculture of stem cells with fetal chondrons using PCL electrospun nanofiber scaffolds. Artificial Cells, Nanomedicine and Biotechnology, 45(2), 283–290. https://doi.org/10.3109/21691401.2016.1146733
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