Two extraction-free reverse transcription loop-mediated isothermal amplification assays for detection of SARS-CoV-2

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Abstract

Background: Current assays for detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) rely on time consuming, costly and laboratory based methods for virus isolation, purification and removing inhibitors. To address this limitation, we propose a simple method for testing RNA from nasopharyngeal swab samples that bypasses the RNA purification step. Methods: In the current project, we have described two extraction-free reverse transcription loop-mediated isothermal amplification (RT-LAMP) assays for the detection of SARS-CoV-2 by using E gene and RdRp gene as the targets. Results: Here, results showed that reverse transcription loop-mediated isothermal amplification assays with 88.4% sensitive (95% CI: 74.9–96.1%) and 67.4% sensitive (95% CI: 51.5–80.9%) for E gene and RdRp gene, respectively. Conclusion: Without the need of RNA purification, our developed RT-LAMP assays for direct detection of SARS-CoV-2 from nasopharyngeal swab samples could be turned into alternatives to qRT-PCR for rapid screening.

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APA

Lai, M. Y., Bukhari, F. D. M., Zulkefli, N. Z., Ismail, I., Mustapa, N. I., Soh, T. S. T., … Lau, Y. L. (2021). Two extraction-free reverse transcription loop-mediated isothermal amplification assays for detection of SARS-CoV-2. BMC Infectious Diseases, 21(1). https://doi.org/10.1186/s12879-021-06876-0

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