Nicotine Quantification In Vitro : A Consistent Dosimetry Marker for e-Cigarette Aerosol and Cigarette Smoke Generation

Abstract

Abstract The e-cigarette category is evolving rapidly, providing consumers with a variety of formats, ranging from cig-a-like products to larger, high-powered modular devices. When generating an in vitro assessment approach across such diverse products, dosimetry considerations are paramount. In this article, we have compared nicotine quantification techniques in two studies using a Vitrocell VC 10 Smoking Robot to generate aerosols from different e-cigarettes. In Study 1, a 3R4F reference cigarette and four different commercially available e-cigarettes were compared: puff-by-puff nicotine concentration was quantified at the same e-cigarette puffing regime (CRM No81) or with different puff durations, (2 or 3 seconds), comparing 3R4F puff-by-puff yields following ISO and HCI smoking regimes. In Study 2, 3R4F and one e-cigarette were assessed for puff-by-puff nicotine concentration in different locations (China and United Kingdom) comparing different nicotine quantification methods with gas chromatography–m...