An improved genome-scale metabolic model of arthrospira platensis C1 (iAK888) and its application in glycogen overproduction

18Citations
Citations of this article
45Readers
Mendeley users who have this article in their library.

Abstract

Glycogen-enriched biomass of Arthrospira platensis has increasingly gained attention as a source for bioethanol production. To study the metabolic capabilities of glycogen production in A. platensis C1, a genome-scale metabolic model (GEM) could be a useful tool for predicting cellular behavior and suggesting strategies for glycogen overproduction. New experimentally validated GEM of A. platensis C1 namely iAK888, which has improved metabolic coverage and functionality was employed in this research. The iAK888 is a fully functional compartmentalized GEM consisting of 888 genes, 1,096 reactions, and 994 metabolites. This model was demonstrated to reasonably predict growth and glycogen fluxes under different growth conditions. In addition, iAK888 was further employed to predict the effect of deficiencies of NO 3− , PO 43− , or SO 42− on the growth and glycogen production in A. platensis C1. The simulation results showed that these nutrient limitations led to a decrease in growth flux and an increase in glycogen flux. The experiment of A. platensis C1 confirmed the enhancement of glycogen fluxes after the cells being transferred from normal Zarrouk’s medium to either NO 3− , PO 43− , or SO 42− -free Zarrouk’s media. Therefore, iAK888 could be served as a predictive model for glycogen overproduction and a valuable multidisciplinary tool for further studies of this important academic and industrial organism.

References Powered by Scopus

The RAST Server: Rapid annotations using subsystems technology

9471Citations
N/AReaders
Get full text

KEGG as a reference resource for gene and protein annotation

4858Citations
N/AReaders
Get full text

Pfam: The protein families database

4856Citations
N/AReaders
Get full text

Cited by Powered by Scopus

High-throughput proteomics and metabolomic studies guide re-engineering of metabolic pathways in eukaryotic microalgae: A review

46Citations
N/AReaders
Get full text

A review on cyanobacteria cultivation for carbohydrate-based biofuels: Cultivation aspects, polysaccharides accumulation strategies, and biofuels production scenarios

43Citations
N/AReaders
Get full text

Influence of carbohydrate additives on the growth rate of microalgae biomass with an increased carbohydrate content

42Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Klanchui, A., Dulsawat, S., Chaloemngam, K., Cheevadhanarak, S., Prommeenate, P., & Meechai, A. (2018). An improved genome-scale metabolic model of arthrospira platensis C1 (iAK888) and its application in glycogen overproduction. Metabolites, 8(4). https://doi.org/10.3390/metabo8040084

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 17

65%

Researcher 6

23%

Professor / Associate Prof. 3

12%

Readers' Discipline

Tooltip

Engineering 6

33%

Biochemistry, Genetics and Molecular Bi... 6

33%

Agricultural and Biological Sciences 4

22%

Chemical Engineering 2

11%

Article Metrics

Tooltip
Mentions
Blog Mentions: 1

Save time finding and organizing research with Mendeley

Sign up for free