Purpose: The purpose of this paper is to study the association between RGD binding kinetics and αvβ3 integrin receptor density in the complex tumor milieu. Procedures: We assessed αvβ3 in vitro and by 68Ga-DOTA- [c(RGDfK)]2 positron emission tomography (PET) in tumors with varying αvβ3. Results: Intrinsic α vβ3 expression decreased in the order of M21⋙MDA-MB-231>M21L in cells. Tumor volume of distribution by PET, V T, was significantly higher in M21 compared to isogenic M21L tumors (0.40±0.01 versus 0.25±0.02; p<0.01) despite similar microvessel density (MVD) likely due to higher αvβ 3. VT for MDA-MB-231 (0.40±0.04) was comparable to M21 despite lower αvβ3 but in keeping with the higher MVD, suggesting superior tracer distribution. Conclusions: This study demonstrates that radioligand binding kinetics of PET data can be used to discriminate tumors with different αvβ3 integrin expression-a key component of the angiogenesis phenotype - in vivo. © 2013 World Molecular Imaging Society.
CITATION STYLE
Alam, I. S., Witney, T. H., Tomasi, G., Carroll, L., Twyman, F. J., Nguyen, Q. D., & Aboagye, E. O. (2014). Radiolabeled RGD tracer kinetics annotates differential αvβ3 integrin expression linked to cell intrinsic and vessel expression. Molecular Imaging and Biology, 16(4), 558–566. https://doi.org/10.1007/s11307-013-0710-3
Mendeley helps you to discover research relevant for your work.