Optimization of Bioactive Metabolite Production from Fusarium oxysporum LBKURCC41

Abstract

Fusarium oxysporum is one of endophyte microbes isolated from dahlia tuber (Dahlia variabilis) which has the ability to produce secondary metabolites. In this research, optimization of chemichal and physical fermentation conditions were carried out. Corn, potato, and sweet potato with particle size of 80 mesh were used as corbon sources to produce secondary metabolite for 5, 10, 15, 20, and 25 days respectively with ethyl acetate was used to extract secondary metabolites. The assessment of antimicrobial activity was then performed against Candida albican, Escherichia coli and Staphylacoccus aureus. A serial of metabolite concentrations 5.7 mg/ml, 3.8 mg/ml, and 1.9 mg/ml were used. The optimum inhibition against microbial pathogen growth was corn for 15 day fementation (C15). The inhibition zone against Candida albicans, E.coli, and S. aureus were 7.62±0.32, 14.15±0.09, and 15.24±0.24 mm respectively at 5.7 mg/ml metabolite concentration. The result of metabolites screening showed overall extract secondary metabolites comprised terpenoid group. The separation of the active ingredients was performed using both Thin Layer Chromatoghraphy and High Performance Liquid Chromatoghraphy (HPLC) depicted 4 component.