Methods of Sperm Vitality Assessment

  • Moskovtsev S
  • Librach C
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Abstract

Sperm vitality is a re fl ection of the proportion of live, membrane-intact spermatozoa determined by either dye exclusion or osmoregulatory capacity under hypo-osmotic conditions. In this chapter we address the two most common methods of sperm vitality assessment: eosin–nigrosin staining and the hypo-osmotic swelling test, both utilized in clinical Andrology laboratories.

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Moskovtsev, S. I., & Librach, C. L. (2013). Methods of Sperm Vitality Assessment (pp. 13–19). https://doi.org/10.1007/978-1-62703-038-0_2

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