DNA Extraction for PCR: Phenol/Chloroform vs. Chelex — A Comparative Study

  • Schnee-Griese J
  • Linder S
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Abstract

Chelex 100 is a chelating resin with high affinity for polyvalent metal ions which can cause degradation of DNA at high temperatures (l). Boiling of DNA in the presence of Chelex prevents the degradation of DNA.

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Schnee-Griese, J., & Linder, S. (1994). DNA Extraction for PCR: Phenol/Chloroform vs. Chelex — A Comparative Study (pp. 167–169). https://doi.org/10.1007/978-3-642-78782-9_38

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