We standardized a method to evaluate the growth kinetics of Mycobacterium tuberculosis by measuring quantitatively the reduction of resazurin by spectrophotometry. Growth curves and the rate of growth of twenty-one M. tuberculosis clinical isolates were determined. The method showed technical simplicity and is inexpensive to assess the fitness of each isolate.
CITATION STYLE
von Groll, A., Martin, A., Portaels, F., da Silva, P. E. A., & Palomino, J. C. (2010). Growth kinetics of mycobacterium tuberculosis measured by quantitative resazurin reduction assay: A tool for fitness studies. Brazilian Journal of Microbiology, 41(2), 300–303. https://doi.org/10.1590/S1517-83822010000200006
Mendeley helps you to discover research relevant for your work.