d-Amino acids play important physiological roles in the mammalian body. Recent investigations revealed that, in mammals, d-amino acids are synthesized from their corresponding l-enantiomers via amino acid racemase. This article describes a method used to measure amino acid racemase activity by high-performance liquid chromatography (HPLC). The assay involves fluorogenic chiral derivatization of amino acids with a newly developed reagent, and enantioseparation of d-and l-amino acid derivatives by HPLC. The method is accurate and reliable, and can be automated using a programmable autosampling injector.
CITATION STYLE
Katane, M., Sekine, M., & Homma, H. (2012). Assay of amino acid racemases. Methods in Molecular Biology, 794, 367–379. https://doi.org/10.1007/978-1-61779-331-8_25
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