Scaffolds for Embryonic Stem Cell Growth and Differentiation

Abstract

This chapter is organized in order to help the reader on choosing materials for scaffolds production, and to serve as a guide for scaffolds preparation and characterization for embryonic stem cell growth and differentiation. First, different methods for the preparation and characterization of scaffolds are briefly described, highlighting critical parameters. Then, we focus on how to evaluate scaffold suitability, such as its stability in culture media and toxicity as well as suitable protocols for sterilization. Qualitative and quantitative analysis of stem cell growth, viability and differentiation by immunofluorescence microscopy and flow cytometry are also presented.