Antibacterial activity of seed kernel extracts of seven mangoes (Mangifera indica) cultivars native to Indonesia against MDR-Pseudomonas aeruginosa isolated from wounds

Abstract

Prastiyanto ME, Darmawati S, Mukaromah AH. 2022. In vitro evaluation of the antibacterial activity of seed kernel extracts of seven mangoes (Mangifera indica) cultivars native to Indonesia against MDR-Pseudomonas aeruginosa isolated from wounds. Biodiversitas 23: 5629-5637. Pseudomonas aeroginusa is the most common bacterium causing wound infections, with the most common solution being antibiotics. However, excessive and inappropriate use of antibiotics will lead to the emergence of multi-drug resistant (MDR) bacterial strains. Therefore, natural ingredients are needed as alternative antibacterial agents. This study aimed to determine the antibacterial activity of seed kernel extracts from seven cultivars of mango (Mangifera indica) from Indonesia, i.e., Cengkir, Kopyor, Golek, Kweni, Avocado, Arumanis, and Manalagi, against MDR-P. aeroginusa bacteria isolated from wounds. The agar well diffusion method was carried out to determine the inhibition zone, and the microdilution method was used to determine the MIC and MBC values. The results showed that the seed kernel extracts of seven cultivars of mangoes had antibacterial activity against MDR-P. aeruginosa. Of the seven mango cultivars, Kweni cultivar seed kernel extracts demonstrated the lowest MIC and MBC values of ≥0.75 mg/mL and ≥12.5 mg/mL. This study concludes that Kweni cultivar seed kernel extracts have the potential to be developed as agents of anti-MDR-P. aeruginosa causes wound infection.