Abstract
The reaction between ethanolamine and sugars in a borate solution gave intense UV absorption at 310 nm. By adding ethanolamine in the mobile phase, simple and sensitive ion-exchange HPLC of sugar-borate complexes was achieved. This method can be used to measure as little as 100 pmole of seven monosaccharides from pectic polysaccharides.
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APA
Nakamura, A., Hatanaka, C., & Nagamatsu, Y. (2000). Ultraviolet spectrometric determination of neutral monosaccharides by HPLC with ethanolamine. Bioscience, Biotechnology and Biochemistry, 64(1), 178–180. https://doi.org/10.1271/bbb.64.178
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