Small molecule-immobilized affinity matrices are indispensable tools in chemical genomics to screen and purify protein targets for biologically active small molecules. Usually, prior to immobilization, small-molecules have to be derivatized at a position that does not significantly abrogate the intrinsic biological activity, or chemically synthesized to have an appropriate functional group for the immobilization chemistry. Here, we describe a photo-cross-linking technique to immobilize biologically active small molecules for protein target screening, without the need for chemical derivatization. © 2012 Springer Science+Business Media, LLC.
CITATION STYLE
Takayama, H., Moriya, T., & Kanoh, N. (2012). Preparation of photo-cross-linked small molecule affinity matrices for affinity selection of protein targets for biologically active small molecules. Methods in Molecular Biology, 800, 75–83. https://doi.org/10.1007/978-1-61779-349-3_6
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