The predominant molecular state of bound enzyme determines the strength and type of product inhibition in the hydrolysis of recalcitrant polysaccharides by processive enzymes

Abstract

Background: Rate-limiting steps in the hydrolysis of recalcitrant polysaccharides by processive enzymes are not known. Results: The predominant molecular state of bound enzyme was revealed by the type and strength of product inhibition. Conclusion: Complexation with the polymer chain is rate-limiting for ChiA, whereas Cel7A is limited by dissociation. Significance Knowledge of rate-limiting steps aids the engineering of better catalysts.