Mitochondrial DNA identified bat species in northeast India: electrocution mortality and biodiversity loss

Abstract

Northeast India with two biodiversity hotspots is recognized as a biodiversity-rich region. However, several extant animals including chiropterans are currently at jeopardy due to habitat loss, electrocution mortality, and other anthropogenic threats. This study examines the efficacy of mitochondrial Cytochrome b (mtCytb) sequences for species-level identification of five electrocuted bat specimens from Manipur state. The similarity search results in the global database, Kimura 2 parameter (K2P) genetic distances, and neighbor-joining (NJ) tree identified all bat specimens into two species, Cynopterus sphinx and Megaerops niphanae. The detection of M. niphanae is the first record of this mammal from the state. In comparison with other Pteropodidae species, the genetic distances clearly discriminate both C. sphinx (7.9–30.2%) and M. niphanae (12.2–25.7%). In addition, the combined tree analysis of present and earlier genetic information of C. sphinx suggested the presence of cryptic lineages and sympatric population in India. This similar approach with more sampling from a wide distribution area could assist the future genetics research on chiropterans and their precise conservation.