We introduce a method by which periplasmic proteins can be released rapidly, simply, and quantitatively by treating cells with chloroform. All the amino acid-binding proteins tested maintained their activity during chloroform treatment. This method makes practical the analysis of the periplasmic protein complement of a large of strains.
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Ferro-Luzzi Ames, G., Prody, C., & Kustu, S. (1984). Simple, rapid, and quantitative release of periplasmic proteins by chloroform. Journal of Bacteriology, 160(3), 1181–1183. https://doi.org/10.1128/jb.160.3.1181-1183.1984