A viral non-coding region determining neuropathogenicity of murine leukemia virus A8 is responsible for envelope protein expression in the rat brain

Abstract

Friend murine leukemia virus clone A8 causes spongiform neurodegeneration in the rat brain. A 0.3-kb fragment containing the R-U5-5′ leader sequence of A8 is required in addition to the A8-env gene to induce spongiosis. The A8-env gene is a primary determinant of neuropathogenicity. Comparative studies of the neuropathogenic virus R7f, which carries the 0.3-kb fragment of A8 and A8-env on the background of the non-neuropathogenic clone 57, and the non-neuropathogenic virus Rec5, which carries A8-env on the background of 57, showed that the 0.3-kb fragment of A8 was responsible for increasing the ratio of Env/Gag expression in the brain, but not in the spleen.