Immunolocalization of the hepatocyte growth factor (HGF) system in the rat ovary and the anti-apoptotic effect of HGF in rat ovarian granulosa cells in vitro

45Citations
Citations of this article
10Readers
Mendeley users who have this article in their library.

Abstract

Hepatocyte growth factor (HGF) regulates granulosa cell (GC) steroidogenesis and suppresses apoptosis in non-ovarian cells. The hypothesis was thus developed that intraovarian HGF supports folliculogenesis by mediating steroidogenesis and suppressing apoptosis. To investigate the latter, the anti-apoptotic actions of HGF were tested in GCs and follicles isolated from immature rats. Results showed that HGF suppressed apoptosis in GC and follicle cultures as visualized using apoptosis indicator dye, YO-PRO-1. Immunohistochemistry was used to investigate the distribution of HGF, c-met, and HGF activator (HGFA) protein during foiliculogenesis in equine chorionic gonadotropin (eCG)-primed rats. Immunoreactive HGF content was the greatest in GCs within preantral follicles. Following eCG, large antral follicles showed elevated HGF staining in theca and interstitial cells when compared with GCs. Intense c-met staining was observed in GCs within non-primed small preantral follicles; following eCG, the level of c-met was diminished in GCs, but increased within theca and interstitial cells. Theca, interstitium, and GCs in non-primed and primed ovaries contained HGFA. Following eCG, HGFA was more apparent in theca cells and the interstitium when compared to that in GCs within large antral follicles. The presence of HGF, c-met, and HGFA in preantral follicles would potentially enable the anti-apoptotic effects of HGF that were observed in vitro to occur in vivo. Advanced folliculogenesis led to a change in the cellular distribution of the HGF, c-met, and HGFA, suggesting that the ovarian HGF system is hormonally regulated in vivo. © 2006 Society for Reproduction and Fertility.

References Powered by Scopus

Apoptosis in the ovary: Molecular mechanisms

407Citations
N/AReaders
Get full text

Role of androgens in follicle maturation and atresia

147Citations
N/AReaders
Get full text

Thecal cell-granulosa cell interactions involve a positive feedback loop among keratinocyte growth factor, hepatocyte growth factor, and Kit ligand during ovarian follicular development

100Citations
N/AReaders
Get full text

Cited by Powered by Scopus

Bone marrow mesenchymal stem cell transplantation improves ovarian function and structure in rats with chemotherapy-induced ovarian damage

174Citations
N/AReaders
Get full text

Human amnion-derived mesenchymal stem cell (hAD-MSC) transplantation improves ovarian function in rats with premature ovarian insufficiency (POI) at least partly through a paracrine mechanism

161Citations
N/AReaders
Get full text

The restorative effects of adipose-derived mesenchymal stem cells on damaged ovarian function

121Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Uzumcu, M., Pan, Z., Chu, Y., Kuhn, P. E., & Zachow, R. (2006). Immunolocalization of the hepatocyte growth factor (HGF) system in the rat ovary and the anti-apoptotic effect of HGF in rat ovarian granulosa cells in vitro. Reproduction, 132(2), 291–299. https://doi.org/10.1530/rep.1.00989

Readers over time

‘12‘15‘18‘20‘22‘23‘2400.751.52.253

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 3

75%

Professor / Associate Prof. 1

25%

Readers' Discipline

Tooltip

Pharmacology, Toxicology and Pharmaceut... 1

25%

Engineering 1

25%

Medicine and Dentistry 1

25%

Veterinary Science and Veterinary Medic... 1

25%

Save time finding and organizing research with Mendeley

Sign up for free
0