Peptidoglycan biosynthesis in Neisseria gonorrhoeae strains sensitive and intrinsically resistant to beta-lactam antibiotics

9Citations
Citations of this article
12Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

Treatment of penicillin-sensitive and intrinsically resistant N. gonorrhoeae strains with their respective inhibitory concentrations of penicillin caused rapid cell death. When the peptidoglycan syntheses of these two strains were examined in the presence of penicillin, the sensitive strain continued to make this cell wall polymer for an extended time, whereas the resistant strain underwent a rapid and marked depression in synthesis. Examination of the labeled sodium dodecyl sulfate-insoluble peptidoglycan made in the presence of inhibitory concentrations of penicillin revealed further differences. The primary effect on the penicillin-sensitive gonococcus was a slight change in peptide cross-linking and a sharp decline in the degree of O-acetylation. In contrast, the resistant strain exhibited a substantial decline in cross-linking, with a very moderate change in O-acetylation. The degree of saturation of the individual penicillin-binding proteins (PBPs) was assessed under these conditions. PBP 2, which exhibits a reduced affinity for penicillin in the resistant strain, appeared to be related to O-acetylation, whereas PBP 1 was implicated in the transpeptidation reaction.

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Dougherty, T. J. (1983). Peptidoglycan biosynthesis in Neisseria gonorrhoeae strains sensitive and intrinsically resistant to beta-lactam antibiotics. Journal of Bacteriology, 153(1), 429–435. https://doi.org/10.1128/jb.153.1.429-435.1983

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 4

44%

Professor / Associate Prof. 2

22%

Researcher 2

22%

Lecturer / Post doc 1

11%

Readers' Discipline

Tooltip

Agricultural and Biological Sciences 2

29%

Biochemistry, Genetics and Molecular Bi... 2

29%

Immunology and Microbiology 2

29%

Computer Science 1

14%

Save time finding and organizing research with Mendeley

Sign up for free