Fungal DNA is present at very low loads in clinical specimens. Molecular detection by amplification assays generally is a challenge because of a potentially multiple input of contaminating DNA from exogenous sources. Besides airborne, handling and cross-contamination, materials and reagents used in the molecular laboratory can contain microbial DNA which is a long underestimated potential source of false positive results. In this contribution decontamination procedures of materials and reagents and the selection of certified microbial DNA-free components for sample collection, DNA extraction, and PCR amplification are discussed with respect to the aim of building up a reliable molecular system for the diagnosis of fungal organisms at the limit of detection.
CITATION STYLE
Lorenz, M. G., Lustig, M., & Linow, M. (2017). Fungal-grade reagents and materials for molecular analysis. In Methods in Molecular Biology (Vol. 1508, pp. 141–150). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6515-1_6
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