Macrophage migration inhibitory factor (MIF) is expressed and released ubiquitously by numerous cell types and tissues. MIF is detected and constitutively expressed at the protein level both intra- and extracellularly. This chapter outlines methods for cultivating, purifying, detecting, and quantifying concentrations of MIF from murine primary derived macrophages and dendritic cell culture supernatants.
CITATION STYLE
Lang, T. (2020). Analysis of Secreted MIF from Cultured Murine Bone Marrow-Derived Immune Cells. Methods in Molecular Biology (Clifton, N.J.), 2080, 39–46. https://doi.org/10.1007/978-1-4939-9936-1_4
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