Recently several authors correlated MBL-2 gene polymorphisms with different pathologies and there is a growing interest for MBL-2 genotyping in a large number of individuals. We have developed a single-tube, rapid, economic, and fully automated melting temperature analysis screening method, based on ABI 7700 Sequence Detection System technology and SYBR Green I chemistry, for the detection of three polymorphisms (exon 1, codons 52, 54, 57) in the MBL-2 gene. We also developed an electronic sheet for the automatic calling of different genotypes, based on the analysis of the first derivative of ABI 7700 raw data.
CITATION STYLE
Hladnik, U., Braida, L., Boniotto, M., Pirulli, D., Gerin, F., Amoroso, A., & Crovella, S. (2002). Single-tube genotyping of MBL-2 polymorphisms using melting temperature analysis. Clinical and Experimental Medicine, 2(2), 105–108. https://doi.org/10.1007/s102380200014
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