Research Development on Vanadium-Dependent Haloperoxidases in Marine Algae. In: Zhang TC., Ouyang P., Kaplan S., Skarnes B. (eds) Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012). Lecture Notes in Electrical Engineerin

Abstract

In order to search for new α-amylase inhibitors from Chinese Yam (Dioscorea opposita Thunb.), the extracts of alkali-soluble crude polysaccharides (ACP), water-soluble crude polysaccharides (WCP), total saponins, and flavones were isolated, and their inhibitory effects on α-amylase were evaluated. The experimental results show that the saponins and flavones had a marked inhibition effect on α-amylase, however, the ACP and WCP exhibited weakly inhibitory activities. The kinetic analysis indicates that the inhibition type of these extracts on α-amylase was competitive, and the enzyme-substrate apparent dissociation constant (Km') of acarbose (the control), WCP, saponins, and flavones is 118.86, 32.46, 79.23, 49.51 mg/ml, respectively. © Springer-Verlag Berlin Heidelberg 2014.