In situ hybridization (ISH) has become a very valuable molecular diagnostic tool to detect specific DNA or RNA sequences in biological samples through the use of complementary DNA- or RNA-labeled probes. Here, we describe an optimized in situ hybridization protocol to detect African swine fever virus (ASFV) DNA in formalin-fixed, paraffin-embedded tissues using digoxigenin-labeled probes.
CITATION STYLE
Ballester, M., & Rodríguez, F. (2014). In situ hybridization with labeled probes: Assessment of African swine fever virus in formalin-fixed paraffin-embedded tissues. Methods in Molecular Biology, 1247, 209–218. https://doi.org/10.1007/978-1-4939-2004-4_16
Mendeley helps you to discover research relevant for your work.