The carbohydrate fraction of microalgal biomass is complex and consists of a combination of neutral, acidic, amino sugars, and sugar alcohols. These carbohydrate amalgamations can be difficult to accurately quantify with the most analytical carbohydrate determination techniques. The method described here provides a straightforward two-step sulfuric acid hydrolysis followed by soluble carbohydrate quantification by either a spectrophotometric method (based on aldehyde functional group derivatization), for an overall combined monomeric sugar concentration determination, or a liquid chromatography method, for a more specific monomeric sugar profile.
CITATION STYLE
Van Wychen, S., & Laurens, L. M. L. (2020). Total Carbohydrate Content Determination of Microalgal Biomass by Acid Hydrolysis Followed by Spectrophotometry or Liquid Chromatography. In Methods in Molecular Biology (Vol. 1980, pp. 191–202). Humana Press Inc. https://doi.org/10.1007/7651_2017_106
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