RNA-Based Assays

Abstract

In addition to DNA-based assays, molecular cytopathology includes RNA-based assays. In routine clinical settings, these assays are used for the detection of relevant biomarkers to refine indeterminate morphological diagnosis and to predict treatment response. In this chapter, we will focus on a wide range of RNA-based techniques, including conventional as well as emerging assays, such as quantitative reverse transcriptase-PCR (qRT-PCR), next-generation RNA sequencing, and multiplex digital color-coded barcode technology. We provide a brief overview of the main technical aspects of RNA workflow analysis with a focus on the potential and the challenges of cytological specimens.