A UHPLC METHOD FOR SERTRALINE DETERMINATION

Abstract

Purpose: To develop and implement a UHPLC method for quantitative determination of sertraline in biological samples - mostly human blood and urine. Material&Methods: Blood and urine samples available from Laboratory of analytical toxicology and Clinic for intensive treatment of acute intoxications and toxicoallergies were used during method validation and case monitoring. Analytical identification of sertraline and/ or metabolites was done by GC-MS. Gas chromatography coupled with flame ionization detection was used for alcohols/volatiles screening of clinical samples. Ultra highperformance liquid chromatography system in tandem with diode-array detector has been used as the main quantitative instrument. Result(s): After critical consideration of available reference data a carefully set of experimental conditions for sertraline extraction and UHPLC determination were adopted and optimized. Preliminary liquid-phase sample purification was applied. Zorbax Extend-C18 column (150 x 4.6 mm, 5 mum) was used under isocratic conditions with phosphate buffer (pH 2.7; 10 mM) containing 1.5 ml L-1 triethylamine - acetonitrile (65:35, v/v) at 20oC, at the flow-rate of 1.0 mL/min and UV detection at 220 nm. This method was validated for the determination of sertraline in human plasma/serum samples (70% recovery). Conclusion(s): A simple yet sensitive and reliable method for sertraline determination was introduced. Linearity over 20-1000 ng mL-1 range was shown; LOQ was 20 ng mL-1. The method was clinically applied for monitoring the blood sertraline levels during a course of detoxication of a female patient.Copyright © 2017, Peytchinski, Gospodin Iliev ET. All rights reserved.