Arohynapenes A and b, new anticoccidial agents produced by penicillium sp. Taxonomy, fermentation, and structure elucidation

Abstract

Penicillium sp. FO-2295, a water isolate, was found to produce a series of new anticoccidial compounds. Two active compounds, designated arohynapenes A and B, were isolated from the fermentation broth of the producing strain by solvent extraction and preparative HPLC. Arohynapene A was deduced to be(2E, 4E)-5-(5-hydroxy-2, 6, 8-trimethyl-5, 6, 7, 8-tetrahydronaphtalene)-2, 4-pe ntadienoic acid, and arohynapene B was (2E, 4E)-5-(2-hydroxymethyl-6, 8-dimethyl-5, 6, 7, 8-tetrahydronaphtalene)-2, 4-p entadienoic acid. Arohynapenes inhibited the growth of Eimeria tenella in an in vitro assay using BHK-21 cells as a host. No schizont in the cells was observed at concentrations ranging above 35.0 μM and 7.0 μM for arohynapenes A and B, respectively. © 1994, JAPAN ANTIBIOTICS RESEARCH ASSOCIATION. All rights reserved.