INVESTIGATION OF TOTAL PHENOLIC CONTENT, FLAVONOID CONTENT, AND HEMOSTATIC ACTIVITY OF BEETROOT (Beta vulgaris. L) EXTRACT IN HEPARIN-INDUCED THROMBOCYTOPENIA RAT

Abstract

The goal of the research was to find out the concentration of phenolic and flavonoid content and also to identify the hemostatic activity of the ethanolic extract of beetroot (Beta vulgaris L) in heparin-induced thrombocytopenia rats. The identification of total phenol content using Folin-Ciocalteu as reagent and flavonoid content were determined by spectrophotometer and continued to the determination of hemostatic activity using heparin as an inducer and then determined the bleeding time, platelet count, and prothrombin time. The results showed that the ethanol extract of beetroot had a total phenolic content of 312.2 mg GAE/g extract and a flavonoid content equivalent to 38.5 mg quercetin equivalent/g. The investigation of bleeding time at doses of 100 mg/kg BW, 200 mg/kg BW, and 400 mg/kg BW was significantly different (p<0.05) compared to the negative group. The dose of 400 mg/kg BW reduced the bleeding time by 5.90±0.11 minutes, which was substantially different from the other doses (p<0.05). At a dose of 400mg/kg BW, the greatest platelet count number was seen, which was statistically different from the control group (p<0.05). The prothrombin Time (PT) values at a dose of 400 mg/kg BW likewise revealed increased activity and were significantly different (p<0.05) from the negative group. The ethanol extract of beetroot (Beta vulgaris L) possesses hemostatic action and is dose-dependent, with a total phenolic concentration of 312.2 mg GAE/g extract and a flavonoid content equivalent to 38.5 mg quercetin equivalent/g extract.