A challenge to the study of regeneration is determining at what point the processes of wound healing and regeneration diverge. The mouse displays level-specific regeneration responses. An amputation through the distal third of the terminal phalanx will prompt a regeneration response and result in a new digit tip that mimics the morphology of the lost digit tip. Conversely, an amputation through the distal third of the intermediate phalanx initiates a wound healing and scarring response. The mouse, therefore, provides a model for studying the transition between wound healing and regeneration in the same animal. This chapter details the methods used in the study of mammalian digit regeneration, including a method to introduce exogenous protein into the mouse digit amputation model via microcarrier beads and methods for analysis of bone regeneration. © 2013 Springer Science+Business Media New York.
CITATION STYLE
Simkin, J., Han, M., Yu, L., Yan, M., & Muneoka, K. (2013). The mouse digit tip: From wound healing to regeneration. Methods in Molecular Biology, 1037, 419–435. https://doi.org/10.1007/978-1-62703-505-7_24
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