We here describe the methods for the isolation of distinct hematopoietic subpopulations, as defined by their immune phenotype by fluorescence-activated cell sorting, and how these cells can be analyzed even at a single-cell level for the gene expression of a number of transcription factors and other differentiation markers. © 2009 Humana Press.
CITATION STYLE
Edvardsson, L., & Olofsson, T. (2009). Real-time PCR analysis for blood cell lineage specific markers. Methods in Molecular Biology, 496, 313–322. https://doi.org/10.1007/978-1-59745-553-4_21
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