The optimization of in vitro micropropagation of betung bamboo (Dendrocalamus asper backer) by medium concentrations and plant growth regulators

Abstract

Bamboo has excellent potential to be developed as a building material, pulp, textile, carbon sink, water-storing, and water-holding. An alternative method for plant propagation is by in vitro micropropagation (tissue culture). The cultured explants were able to grow to be a new individual having shoots and leaves. Murashige and Skoog (MS) medium concentrations solely were significantly affected by the number of shoots. The addition of thidiazuron (TDZ) into media resulted in the best percentage of shoot number (80%) in all concentrations of media. Using low concentration, TDZ was able to produce more leaves than other cytokinins. The appropriate concentration of MS medium and the plant growth regulator for Betung bamboo's tissue culture is 3/4 MS with the addition of 3 ppm TDZ.