Physiological Intracytoplasmic Sperm Insemination Based on Hyaluronic Acid-Binding Ability

Abstract

This comprehensive, up-to-date text, which brings together the key practical elements of the rapidly evolving field of sperm DNA and chromatin abnormalities, is divided thematically into five main sections. Part I discusses human sperm chromatin structure and nuclear architecture, while part II presents laboratory evaluation of sperm DNA damage, including SCSA, SCD, TUNEL and Comet assays, and cytochemical tests. Biological and clinical factors in the etiology of sperm DNA damage are discussed in part III, including oxidative stress, abortive apoptosis, cancer, and environmental and lifestyle factors. Part IV presents clinical studies on the utility of sperm DNA damage tests, both with natural and ART-assisted pregnancies, and debates the clinical utility of such tests. Finally, part V discusses current treatment options, such as antioxidant therapy, varicocelectomy, advanced sperm processing techniques and the use of testicular sperm. We are now beginning to better understand the unique organization of the sperm chromatin, as well as the nature and etiology of sperm DNA damage. Written and edited by worldwide experts in andrology, A Clinician's Guide to Sperm DNA and Chromatin Damage is an excellent resource for reproductive medicine and REI specialists, urologists, reproductive biologists and any professional working with the infertile male . Intro; Foreword; Preface; Contents; Editors; Contributors; Part I: Human Sperm Chromatin Structure; Chapter 1: Sperm Chromatin: An Overview; 1.1 Sperm Chromatin: The First 50 Years; 1.2 Spermatogenesis: Terminal Differentiation and Reprogramming of the Testicular Cell Genome; 1.3 Chromatin Reorganization in Maturing Spermatids; 1.4 Protamines P1 and P2; 1.5 Protamine-DNA Interactions and Structure of the Complex; 1.6 Higher-Ordered Organization of Chromatin in Mature Sperm; 1.7 Preservation of DNA Domains and Nuclear Matrix Associations 1.8 Reactivation of Paternal Chromatin Following Sperm-­Oocyte Fusion1.9 Consequences of Disrupting Sperm Chromatin Remodeling; References; Chapter 2: Sperm Nucleoproteins (Histones and Protamines); 2.1 Introduction; 2.2 Nucleoprotamine Complex in Sperm; 2.2.1 Protamine Post-translational Modifications; 2.2.2 Protamine Alterations in Infertile Patients; 2.3 Nucleohistone Complex in Sperm; 2.3.1 Histone Variants; 2.3.2 Histone Post-translational Modifications; 2.3.3 Histone-Bound Sperm Chromatin; 2.3.4 Histone Alterations and Male Infertility; 2.4 Concluding Remarks; References Cha…