The rapid and accurate quantification of peptides is a critical element of modern proteomics that has become increasingly challenging as proteomic data sets grow in size and complexity. We present here FlashLFQ, a computer program for high-speed label-free quantification of peptides and proteins following a search of bottom-up mass spectrometry data. FlashLFQ is approximately an order of magnitude faster than established label-free quantification methods and can quantify data-dependent analysis (DDA) search results from any proteomics search program. It is available as a graphical user interface program, a command line tool, a Docker image, and integrated into the MetaMorpheus search software.
CITATION STYLE
Millikin, R. J., Shortreed, M. R., Scalf, M., & Smith, L. M. (2023). Fast, Free, and Flexible Peptide and Protein Quantification with FlashLFQ. In Methods in Molecular Biology (Vol. 2426, pp. 303–313). Humana Press Inc. https://doi.org/10.1007/978-1-0716-1967-4_13
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