Development and validation of a UV spectrophotometric method for determination of diacerein in bulk and a capsule dosage form

Abstract

A simple, accurate and selective UV-spectrophotometric method was developed for the estimation of diacerein in bulk and pharmaceutical dosage forms. The method was developed and validated according to International Conference on Harmonization (ICH Q2 R1) guidelines. The developed method was validated statistically with respect to linearity, range, precision, accuracy, ruggedness, limit of detection and limit of quantitation. The study was carried out in citrate buffer pH 6.0 and λ max was found to be 258.8 nm. Pure drug concentration was prepared in the range of 1-10 μg/ml and the linear regression analysis data showed good linear relationship with an R 2 value of 0.999. The limit of detection and limit of quantitation were found to be 0.675 and 1.189 μg/ml, respectively. Recoveries were found to be in the range of 100.815 to 101.744 % and % RSD was less than 2 %, which indicated that the developed method was accurate, precise, specific, rapid and suitable for the analysis of commercial samples.