Breeding potential of introgression lines developed from interspecific crossing between upland cotton (Gossypium hirsutum) and Gossypium barbadense: Heterosis, Combining Ability and Genetic Effects

Abstract

Upland cotton (Gossypium hirstumL.), which producesmore than 95% of the world natural cotton fibers, has a narrow genetic base which hinders progress in cotton breeding. Introducing germplasm from exotic sources especially from another cultivated tetraploid G. barbadense L. can broaden the genetic base of Upland cotton. However, the breeding potential of introgression lines (ILs) in Upland cotton with G. barbadense germplasmintegration has not been well addressed. This study involved six ILs developed from an interspecific crossing and backcrossing between Upland cotton and G. barbadense and represented one of the first studies to investigate breeding potentials of a set of ILs using a full diallel analysis. Highmid-parent heterosis was detected in several hybrids between ILs and a commercial cultivar, which also outyielded the high-yielding cultivar parent in F<1<, F<2< and F<3< generations. A further analysis indicated that general ability (GCA) variance was predominant for all the traits, while specific combining ability (SCA) variance was either non-existent or much lower than GCA. The estimated GCA effects and predicted additive effects for parents in each trait were positively correlated (at P<0.01). Furthermore, GCA and additive effects for each trait were also positively correlated among generations (at P<0.05), suggesting that F<2< and F<3< generations can be used as a proxy to F<1< in analyzing combining abilities and estimating genetic parameters. In addition, differences between reciprocal crosses in F<1< and F<2< were not significant for yield, yield components and fiber quality traits. Butmaternal effects appeared to be present for seed oil and protein contents in F<3<. This study identified introgression lines as good general combiners for yield and fiber quality improvement and hybrids with high heterotic vigor in yield, and therefore provided useful information for further utilization of introgression lines in cotton breeding.